LD KIT- LEUKOCYTE DETECTION KIT
The most common type of WBC in semen are polymorphonuclear or granulocytes (50–60%), followed by macrophages (20–30%) and T-lymphocytes (2–5%).
Reproductive tract infection is an important cause of male infertility, and anti-inflammatory treatment is an important means to improve male fertility.
The increase of leukocytes in semen may be a sign of reproductive system infection. When the concentration of leukocytes is more than 1 × 106/mL, sperm quality will decrease.
Leukocytes had a positive association with normal morphology and progressive motility in semen samples at a concentration of 0-1 × 10(6)/mL. The findings suggest that the association between leukocytes (PMNL) and semen quality might be concentration dependent. More than this range leukocytes negatively impact on semen quality as a result of the presence of reactive oxygen species (ROS) that ROS are harmful to spermatozoa.
Leukocytospermia, defined by the World Health Organization (WHO) as more than 1 million leukocytes per milliliter, has an incidence of 10-20% in the general population and is especially common in infertile men. However, lower concentrations of seminal leukocytes (0-1 × 106/mL) are still more widespread, and are seen even in the absence of infection .
Storage conditions
LD kit should be kept out of sun and stored at the temperature of 2~8° C for 6 months.
Sperm sample
Semen samples should be collected in a sterile container. The staining sperms should be performed. Fresh sample is needed.
Instruction for use
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