SCMA kit (sperm chromatin maturation assay kit)

Routine semen analysis is the basic analysis in the exploration of male infertility. It provides useful data concerning sperm count, sperm motility and viability, sperm morphology, performance of genital glands, and ejaculation. Although, the contribution of the semen analysis is limited on the assessment of some important criteria implicated in the sperm functional potential. In fact, it is well established that the maturity of sperm chromatin is essential for fertilizing capacity of spermatozoa and embryonic development.

SCMA KIT ( sperm chromatin maturation assay kit) evaluate the maturation rate of sperms. Maturation affect on fertility rate.

The maturity of sperm chromatin is essential for fertilizing capacity of spermatozoa and embryonic development Aniline blue (AB) test evaluates the degree of sperm chromatin compaction or maturation, and it is able to detect sperm chromatin defects related to their nucleoprotein content. This method allows discriminating between the presence of lysine-rich histones and arginine- and cysteine-rich protamine in sperm nuclei. Consequently, histone-rich nuclei of immature sperm will take up the blue stain whereas mature protamine-rich nuclei remain unstained.

Store condition 

After receiving the SCMA kit store at 2-8 º for 12 months.

Sperm sample

Semen samples should be collected in a sterile container. The staining sperms should be performed. Maturation assay test does not require a fresh sample.